Master r-DNA Tools: Electrophoresis, Vectors & Ligase



🔴 LIVE: Biotechnology: Principles and Processes | Gel Electrophoresis, Cloning Vectors & DNA Ligase | Class 12 Biology & NEET Preparation with Bindiya Aggarwal Mam.

In this live session, we dive deep into the essential Tools of Recombinant DNA Technology from Chapter 9 (Biotechnology: Principles and Processes). Master the high-yield NCERT concepts, diagrams, and past year exam questions!

📌 Topics Covered in Today’s Live Class:
• Gel Electrophoresis: Principle, Agarose Gel, Ethidium Bromide & UV visualisation, Elution
• Cloning Vectors: Origin of Replication (ori), Selectable Markers (ampR, tetR), Cloning Sites (pBR322)
• Insertional Inactivation & Alternative Selectable Markers (Beta-galactosidase)
• Vectors for Cloning in Plants & Animals (Agrobacterium tumefaciens & Retroviruses)
• DNA Ligase: The Molecular Glue (Phosphodiester Bond formation)
• NCERT Line-by-Line Breakdown & High-Yield NEET / CBSE PYQs

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